Defining a new role of GW182 in maintaining miRNA stability.
نویسندگان
چکیده
GW182 binds to Argonaute (AGO) proteins and has a central role in miRNA-mediated gene silencing. Using lentiviral shRNA-induced GW182 knockdown in HEK293 cells, this study identifies a new role of GW182 in regulating miRNA stability. Stably knocking down GW182 or its paralogue TNRC6B reduces transfected miRNA-mimic half-lives. Replenishment of GW182 family proteins, as well as one of its domain Δ12, significantly restores the stability of transfected miRNA-mimic. GW182 knockdown reduces miRNA secretion via secretory exosomes. Targeted siRNA screening identifies a 3'-5' exoribonuclease complex responsible for the miRNA degradation only when GW182 is knocked down. Immunoprecipitation further confirms that the presence of GW182 in the RISC complex is critical in protecting Argonaute-bound miRNA.
منابع مشابه
Defining a novel role of GW182 in maintaining miRNA stability
As you will see, while the referees acknowledge that the finding that GW182 prevents miRNA degradation is potentially interesting, both referees 1 and 2 also point out that, as it stands, the manuscript is rather preliminary and requires significant strengthening before it can be considered for publication by EMBO reports. Both referees remark that endogenous miRNA levels need to be examined in...
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عنوان ژورنال:
- EMBO reports
دوره 13 12 شماره
صفحات -
تاریخ انتشار 2012